Frequently Questions

Bacteria DNA Extraction Kit

Make sure that you use the recommended volume of bacterial culture for extraction. The culture should be fresh or properly stored one. Also ensure that the right amount of Lysis buffer and Proteinase K are added.

Lower culture density – make sure that the culture OD reached 1.0 before processing the experiment.

The genomic DNA was handled improperly – Pipetting steps should be handled as gently as possible.

Improper storage and age of sample – Avoid using too old bacterial culture or stored at suboptimal conditions. Sheared DNA may be obtained from the old bacterial samples. Fresh samples are recommended for maximum genomic DNA yield

Frequent freeze thaw of DNA – avoid frequent freeze thaw of eluted DNA

Non sterile consumables usage – ensure that the pipettes are cleaned, vials and tips autoclaved properly. Otherwise, the DNA will degrade due to nuclease activity.

Improper Agarose gel and buffers – make sure that the gel and buffers are prepared properly. Do not use the old or used Agarose gels for checking the DNA

The eluted DNA smells ethanol.
DNA floats back from the well of Agarose gels even if the DNA is loaded with loading dye.
DNA will not freeze well at -200C

You can measure DNA by NanoDrop spectrophotometry or by fluorimetry using Qubit. In Qubit assay, the dye binds specifically to double stranded DNA and not to nucleotides like single-stranded DNA, or RNA.

Qubit assay specifically measures double stranded DNA and therefore provides a more accurate detection of DNA concentration and amount. NanoDrop spectrophotometry measures DNA concentration by determining the absorbance of light at 260 nm. Though double-stranded DNA has an absorbance at this wavelength, other molecules also absorb including proteins, RNA, single-stranded DNA, free nucleotides, phenol, and other contaminants. Therefore, NanoDrop measurements are prone to over estimation of DNA amounts due to the detection of contaminants in the sample.

The XpressDNA Bacteria kit works well with fresh and properly stored gram positive and gram negative bacterial cultures.

Yes, the DNA can be used for library preparation and sequencing. The protocol is optimized to extract DNA without contaminating proteins and PCR inhibitors.

No, you can directly go for downstream applications like PCR, restriction digestion, etc.,

Yes. Avoid using Tris-EDTA (TE) buffer as EDTA will inhibit the activity of enzymes used in a downstream process such as PCR, restriction digestion, etc.

The XpressDNA Bacteria kits are produced using magnetic nanoparticles based patented technology. The major advantages include lesser dependence on time-consuming steps like centrifugation, use of spin columns and multiple tube changes. The recovery of genomic DNA is higher because of the increased surface area of the nanoparticles.

The reagents present in this kit are guaranteed to be stable over a period of one year with the proper handling and storage condition.

Blood Mini Kit

Make sure that you use the recommended volume of properly stored blood for extraction. Also ensure that the right amount of Lysis buffer and Proteinase K are added.

  • The genomic DNA was handled improperly – Pipetting steps should be handled as gently as possible.
  • Improper storage of sample – Avoid using blood which is clotted or stored at suboptimal conditions.
  • The eluted DNA smells ethanol.
  • DNA floats back from the well of agarose gels even if the DNA is loaded with loading dye.
  • DNA will not freeze well in -20 ℃

You can measure DNA by NanoDrop spectrophotometry or by fluorimetry using Qubit. In Qubit assay, the dye binds specifically to double-stranded DNA and not to nucleotides, single-stranded DNA, or RNA.

Qubit assay specifically measures double-stranded DNA and therefore provides a more accurate detection of DNA concentration and amount. NanoDrop spectrophotometry measures DNA concentration by determining the absorbance of light at 260 nm. Though double-stranded DNA has an absorbance at this wavelength, other molecules also absorb including proteins, RNA, single-stranded DNA, free nucleotides, phenol, and other contaminants. Therefore, NanoDrop measurements are prone to overestimation of DNA amounts due to the detection of contaminants in the sample.

The XpressDNA Blood Mini kit works well with fresh, old and /or haemolysed blood. The kit is ideal for isolating genomic DNA from blood samples stored in EDTA K2/K3, Heparin and Sodium fluoride storage vials.

No. This kit is not recommended for DNA extraction from blood clots.

Yes. Avoid using Tris-EDTA (TE) buffer as EDTA will inhibit the activity of enzymes used in downstream process such as PCR, restriction digestion, etc.

The XpressDNA Blood kits are produced using a magnetic nanoparticles based patented technology. The major advantages include lesser dependence on time consuming steps like centrifugation, use of spin columns and multiple tube changes. The recovery of genomic DNA is higher because of the increased surface area of the nanoparticles.

The reagents present in this kit are guaranteed to be stable over a period of one year.

Glutathione-Magnetic Nanoparticles

The maximum binding capacity of Glutathione-magnetic nanoparticles is approximately 2mg of GST-fusion protein per ml of the product.

Glutathione-magnetic nanoparticles are currently available as 1ml and 5ml products.

MagGenome’s Glutathione-Magnetic nanoparticles have significantly higher binding capacity compared to most of the Glutathione-magnetic beads available in the market. The protocol is quick and simple because all washing and elution steps are performed with the help of an external magnetic field. It is ensured that the loss of the affinity resin is nil during the process compared to Sepharose beads. Non-specific protein binding is also significantly low.

The recommended storage condition of the product is 4℃. The product is stable for one year under ideal storage conditions

Purification of GST tagged fusion proteins.

Plasmid DNA Extraction Kit

Make sure that you take recommended volume of bacterial culture for extraction. The culture should be fresh. Also ensure that the exact amount of extraction buffer was added.

Lower culture density – make sure that the culture OD600nm reached 1 – 1.5 before processing the experiment.

The Plasmid DNA was handled improperly – Pipetting steps should be handled as gently as possible.

Avoid too much of frothing while pipette mixing

Do not vortex the assay tube at any stage of extraction

Prolonged incubation at 80 ℃ while elution

Improper storage and age of sample – Avoid using too old bacterial culture or culture stored at suboptimal conditions. Sheared DNA may be obtained from the old bacterial samples. Fresh samples are recommended for maximum plasmid DNA yield.

Frequent freeze thaw of DNA – avoid frequent freeze thaw of eluted plasmid DNA

Non sterile consumables usage – ensure that the pipettes are cleaned, vials and tips autoclaved properly. Otherwise, the DNA will degrade due to nuclease activity.

Improper Agarose gel and buffers – make sure that the gel and buffers are prepared properly. Do not use the old or used Agarose gels for checking the plasmid DNA

The eluted DNA smells ethanol.

DNA floats back from the well of Agarose gels even if the DNA is loaded with loading dye.

DNA will not freeze well at -20 ℃

You can measure DNA by NanoDrop spectrophotometry

The XpressDNA Plasmid kit works well with fresh bacterial cultures containing clones.

Ensure that lysis incubation time is followed accurately, if prolonged incubation happened at 80°C during lysis time, will lead to genomic DNA contamination.

Use fresh bacterial culture pellets for extraction.

Check whether the culture contains plasmid. Ensure that the cells are properly transformed.

Ensure that ethanol was added in the Wash buffer

Ensure the temperature of water bath is 80°C

Generally plasmid has three bands like supercoiled, linear and open circular forms.

Ensure that the cells are properly transformed with single plasmid only.

Avoid harsh pipette mixing while doing extraction, washing and elution.

Avoid very old cultures

Yes, the Plasmid DNA can be used for sequencing. The protocol is optimized to extract DNA without contaminating proteins and PCR inhibitors.

No, you can directly go for downstream applications like PCR, restriction digestion, transformation etc.,

Yes. Avoid using Tris-EDTA (TE) buffer as EDTA will inhibit the activity of enzymes used in a downstream process such as PCR, restriction digestion, etc.

The XpressDNA Plasmid kits are produced using magnetic nanoparticles based patented technology. The major advantages include lesser dependence on time-consuming steps like centrifugation, use of spin columns and multiple tube changes. The recovery of plasmid DNA is higher because of the increased surface area of the nanoparticles.

The reagents present in this kit are guaranteed to be stable over a period of one year under the proper handling and storage condition.

Protein A-Magnetic Nanoparticles

Protein A and Protein G can bind to IgGs, but their binding properties differ among species and subclasses of IgG.
-Protein A is preferred for human, rabbit, pig, dog, and cat IgG.
-Protein G has the better binding capacity for a broader range of mouse, rat and human IgG subclasses.

The maximum binding capacity of Protein A-magnetic nanoparticles is approximately 150μg IgG per 1 mg of the nanoparticles.

Protein A-magnetic nanoparticles are currently available as 1ml and 5ml products.

MagGenome’s Protein A-Magnetic nanoparticles have significantly higher binding capacity compared to most of the Protein A-magnetic beads available in the market. The protocol is quick and simple because all washing and elution steps are performed with the help of an external magnetic field. It is ensured that the loss of the affinity resin is nil during the process unlike Sepharose beads.

The recommended storage condition of the product is 4 ℃ .The product is stable for one year under ideal storage conditions.

Small scale antibody purification, Immunoprecipitation, Chromatin immunoprecipitation, RNA-IP.

Protein G-Magnetic Nanoparticles

Protein A and Protein G can bind to IgGs, but their binding properties differ among species and subclasses of IgG.
-Protein A is preferred for human, rabbit, pig, dog, and cat IgG.
-Protein G has a better binding capacity for a broader range of mouse, rat, and human IgG subclasses.

The maximum binding capacity of Protein G-magnetic nanoparticles is approximately 120-150μg IgG per 1 mg of the nanoparticles.

Protein G-magnetic nanoparticles are currently available as 1ml and 5ml products.

MagGenome’s Protein G-Magnetic nanoparticles have significantly higher binding capacity compared to most of the Protein G-magnetic beads available in the market. The protocol is quick and simple because all washing and elution steps are performed with the help of an external magnetic field. It is ensured that the loss of the affinity resin is nil during the process compared to Sepharose beads.

The recommended storage condition of the product is 40 ℃. The product is stable for one year under ideal storage conditions.

Small scale antibody purification, Immunoprecipitation, Chromatin immunoprecipitation, RNA-IP

Saliva DNA Extraction Kit

Make sure that you use the recommended volume of saliva for extraction. The saliva should be fresh or properly stored at -200C. Also ensure that the correct amount of Lysis buffer and Proteinase K are added.

The genomic DNA was handled improperly – Pipetting steps should be handled as gently as possible.

Make sure that saliva treated with stabilization buffer properly.

Improper storage and age of sample – Avoid using too old saliva or stored at suboptimal conditions. Sheared DNA may be obtained from the old samples.

Frequent freeze thaw of DNA – avoid frequent freeze thaw of eluted DNA.

Non sterile consumables usage – ensure that the pipettes are cleaned, vials and tips autoclaved properly. Otherwise, the DNA will degrade due to nuclease activity.

Improper Agarose gel and buffers – make sure that the gel and buffers are prepared properly. Do not use the old or used Agarose gels for checking the DNA

The eluted DNA smells ethanol.

DNA floats back from the well of Agarose gels even if the DNA is loaded with loading dye.

DNA will not freeze well at -20 ℃

You can measure DNA by NanoDrop spectrophotometry or by fluorimetry using Qubit. In Qubit assay, the dye binds specifically to double stranded DNA and not to nucleotides like single-stranded DNA, or RNA.

Qubit assay specifically measures double stranded DNA and therefore provides a more accurate detection of DNA concentration and amount. NanoDrop spectrophotometry measures DNA concentration by determining the absorbance of light at 260 nm. Though double-stranded DNA has an absorbance at this wavelength, other molecules also absorb including proteins, RNA, single-stranded DNA, free nucleotides, phenol, and other contaminants. Therefore, NanoDrop measurements are prone to over estimation of DNA amounts due to the detection of contaminants in the sample.

The XpressDNA Saliva kit works well with fresh and properly stored saliva at -200C.

Yes, the DNA can be used for library preparation and sequencing. The protocol is optimized to extract DNA without contaminating proteins and PCR inhibitors.

No, you can directly go for downstream applications like PCR, restriction digestion, etc.,

Yes. Avoid using Tris-EDTA (TE) buffer as EDTA will inhibit the activity of enzymes used in a downstream process such as PCR, restriction digestion, etc.

The XpressDNA Saliva kits are produced using magnetic nanoparticles based patented technology. The major advantages include lesser dependence on time-consuming steps like centrifugation, use of spin columns and multiple tube changes. The recovery of genomic DNA is higher because of the increased surface area of the nanoparticles.

The reagents present in this kit are guaranteed to be stable over a period of one year with the proper handling and storage condition.

Tissue/Cell line Mini Kit

Make sure the appropriate amount of tissue is used for extraction. The tissue has to be completely homogenized. Also ensure that the right amount of Lysis buffer and Proteinase K are added.

  • The genomic DNA was handled improperly – Pipetting steps should be handled as gently as possible.
  • Improper storage of sample – Repeated freezing and thawing of stored samples should be avoided as this may lead to shearing. Follow the storage conditions recommended in the user’s manual.
  • The sample is old – Sheared DNA may be obtained from the old tissue or cell samples. Fresh samples are recommended for maximum genomic DNA yield
  • The eluted DNA smells ethanol.
  • DNA floats back from the well of agarose gels even if the DNA is loaded with loading dye.
  • DNA will not freeze well in -20℃

You can measure DNA by NanoDrop spectrophotometry or by fluorimetry using Qubit. In Qubit assay, the dye binds specifically to double-stranded DNA and not to nucleotides, single-stranded DNA, or RNA.

Qubit assay specifically measures double stranded DNA and therefore provides a more accurate detection of DNA concentration and amount. NanoDrop spectrophotometry measures DNA concentration by determining the absorbance of light at 260 nm. Though double stranded DNA has the absorbance at this wavelength, other molecules also absorb including proteins, RNA, single stranded DNA, free nucleotides, phenol, and other contaminants. Therefore, NanoDrop measurements are prone to overestimation of DNA amounts due to the detection of contaminants in the sample.

So far, we and our customers have successfully extracted and amplified DNA from animal liver, spleen, kidney, heart, lungs, muscle, rat tail, fish muscle and ethanol preserved fish tissues. Also this kit is ideal for extracting DNA from human tumor tissues and cell lines.

Yes, the DNA can be used for library preparation and sequencing. The protocol is optimized to extract DNA without contaminating proteins and PCR inhibitors.

Yes. Avoid using Tris-EDTA (TE) buffer as EDTA will inhibit the activity of enzymes used in a downstream process such as PCR, restriction digestion, etc.

No. This kit is not recommended for DNA extraction from FFPE samples.

The XpressDNA tissue/cell line kits are produced using magnetic nanoparticles based patented technology. The major advantages include lesser dependence on time-consuming steps like centrifugation, use of spin columns and multiple tube changes. The recovery of genomic DNA is higher because of the increased surface area of the nanoparticles.

The reagents present in this kit are guaranteed to be stable over a period of one year.