Bacterial DNA Isolation Kit – XpressDNA Bacteria Kit 250Rxn
The MagGenome XpressDNA Isolation Kit is optimized for genomic DNA purification, providing a simple and efficient method of genomic bacterial DNA isolation from both Gram-negative and Gram-positive bacterial cells. Magnetic Nanoparticles provide large surface area and highly specific nucleic acid binding capacity resulting in purification of high quality and quantity of double stranded genomic DNA from various bacterial strains. The protocol does not require phenol/chloroform or alcohol precipitation. The purified DNA is approximately 20 – 40 kb in size with highest integrity, double strand ratio and is suitable for downstream applications like NGS, Southern blot, SNP genotyping, cloning, sequencing, restriction enzyme digestion and PCR or other enzymatic reactions without any further purification.
XpressDNA Bacteria Kit Advantages
- Ready to go for sequencing without further purification
- High quality intact gDNA
- Limited centrifugation steps
- No phenol or chloroform usage
- Wide compatibility with a variety of bacterial strains and mycobacterium
- Non-toxic and easy storage of kit reagents
The kit contains Proteinase K powder along with its respective dilution buffer. Customer has to disperse the Proteinase K in the given buffer. The enzymes (Proteinase K and RNase) has to be stored at 2 – 8°C. All other solutions should be kept tightly sealed and stored at room temperature.
The XpressDNA Bacteria Kit is shipped at room temperature and on arrival enzymes should be stored at 4ºC.
XpressDNA Bacteria Kit Contents:
- Bacteria Enzyme buffer
- Bacteria Lysis buffer
- Bacteria MagNa Mix
- Bacteria Wash Buffer 1
- Bacteria Wash Buffer 2
- Bacteria Elution Buffer
- Proteinase K Enzyme lyophilized
- Proteinase K dilution buffer
- Bacteria RNase A solution
- MagNa stand (optional)
Reference Guide Bacteria 2019
Reference Guide Bacteria DNA from Infected Leaf Kit 2020