Description
Description
The XpressDNA Blood kit provides a simple and quick system to isolate high quality genomic DNA
from blood, whether fresh or aged. The XpressDNA technology provides an easy and flexible Magnetic Nanoparticles based method that allows the extraction of high quantities of pure DNA as evident from the 260/280 ratio and the agarose gel electrophoresis. The purity ratio of DNA ranges from 1.75 – 1.8 (260/280) and 1.8 – 2.0 (260/230). DNA extracted using XpressDNA Blood kit is amenable for all downstream applications such as PCR, Real time PCR, Restriction digestion, Sanger sequencing and NGS. XpressDNA Blood kit guarantees extraction of high quality genomic DNA in better yields compared to other commercially available kits from a starting blood volume of 0.2 – 0.4ml. The kit can be used for extraction of DNA from:
Fresh, frozen or aged blood
Blood stored in different storage vials (EDTA, citrate, heparin and fluoride)
PBMCs
Umbilical cord blood
Blood clot
XpressDNA Blood Mini Kit Advantages
Uses Magnetic Nanoparticle based technology for extraction
High quality DNA obtained in less amount of time and very few centrifugation steps
Gives good results even with blood clot/ aged blood/ blood spots
The kit is compatible with any type of vacutainer (Citrate/EDTA/fluoride/heparin tubes)
Amenable for various downstream processing like PCR and sequencing
XpressDNA Blood Mini Kit Contents:
• Blood Lysis Buffer -1
• Solution A
• Blood Lysis Buffer-2
• Blood MagNa mix
• Blood Wash Buffer-1
• Blood Wash Buffer-2
• Proteinase K Powder & Dilution Buffer
• Blood Elution buffer
• MagNa stand (optional)
Shipping Condition
The XpressDNA Blood Mini Kit is shipped at room temperature and on arrival enzymes should be stored at 4ºC.
Requirements (Reagents & Equipment)
Customer-Supplied Reagents and Equipment
Benchtop microcentrifuge
Water bath
96-100 % ethanol
Pipettes
Microcentrifuge tubes
Vortex
For Protocol visit Resources -> Reference Guide
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