XpressDNA Mycobacterium Kit 250 rxn
The MagGenome XpressDNA Mycobacterium Kit is optimized for genomic DNA purification, providing a simple and efficient method of genomic DNA extraction from MTB cells. Magnetic Nanoparticles provide large surface area and highly specific nucleic acid binding capacity resulting in purification of high quality and quantity of double stranded genomic DNA from various bacterial strains. The protocol does not require phenol/chloroform or alcohol precipitation. The purified DNA is approximately 20 – 40 kb in size with highest integrity, double strand ratio and is suitable for downstream applications like NGS, Southern blot, SNP genotyping and PCR or other enzymatic reactions without any further purification.
XpressDNA Mycobacterium Kit Advantages
• Ready to go for sequencing without further purification
• High quality intact gDNA
• Limited centrifugation steps
• No phenol or chloroform usage
• Wide compatibility with a variety of mycobacterium strains
• Non-toxic and easy storage of kit reagents
The kit contains Proteinase K powder along with its respective dilution buffer. Customer has to disperse the Proteinase K in the given buffer. The enzymes (Proteinase K and RNase) has to be stored at 2 – 8°C. All other solutions should be kept tightly sealed and stored at room temperature.
The XpressDNA Bacteria Kit is shipped at room temperature and on arrival enzymes should be stored at 4ºC.
XpressDNA Bacteria Kit Contents:
• MTB Enzyme buffer
• MTB Lysis buffer
• MTB MagNa Mix
• MTB Wash Buffer 1
• MTB Wash Buffer 2
• MTB Elution Buffer
• Proteinase K Enzyme lyophilized
• Proteinase K dilution buffer
• MTB RNase A solution
• MagNa stand (optional)
Reference Guide Mycobacterium 2019