This kit is designed for automated or manual extraction of high-quality DNA from blood with a starting volume of 100-200 μl with an approximate yield of ≥ 5 μg of DNA.
You can use the kit for manual extraction or in automation with the instruments and workstations such as Kingfisher Flex, Zybio and Alta where it is capable of processing 1-96 samples in a single run. The Kit provides patented magnetic nanoparticle-based DNA purification from whole blood samples.
This DNA isolation Kit is based on a direct blood lysis method that consequently involves minimal handling and effective removal of contaminants and inhibitors.
DNA extracted using XpressAutomag Blood DNA Kit is amenable for all downstream applications. You can use the DNA directly in applications such as PCR, Real-time PCR, restriction digestion, and sequencing applications.
Product Specifications | |
Parameter | Specifications |
Sample Type | Fresh/ frozen/ or aged blood samples, blood stored in different vacutainers (EDTA, Heparin, Citrate, and Fluoride) |
Instrumentation Required | Automation ( Kingfisher Flex, Zybio and Alta etc.) or manual extraction using magnetic separation stand |
Sample Volume needed | 200 μl |
Sample run time | 30-45 minutes |
Yield | 5-30 μg |
Samples per run | 1-96 samples per run. |
Elution volume | 30-100 μl |
Downstream applications | PCR, Real-time PCR, restriction digestion, and sequencing applications. |
Performance and Data
Extraction of DNA from different blood storage vacutainers
Table 1: Nanodrop data of the gDNA extracted from different blood storage vacutainers
Storage | Conc. (ng/μl) | A260/280 | A260/230 | Yield (μg) |
EDTA | 118.3 | 1.93 | 1.85 | 5.92 |
Citrate | 120.6 | 1.93 | 1.85 | 6.03 |
Heparin | 116.2 | 1.94 | 1.77 | 5.81 |
Fluoride | 127.7 | 1.93 | 1.84 | 6.39 |
Do also consider trying out our XpressDNA Blood DNA Extraction Kit — ideal for a large variety of blood samples such as stored blood, PBMCs, umbilical cord blood and blood clots. .
> For FTA cards and dried blood spots, you can use our XpressDNA Dried Blood Spot Kit.