XpressPure NGS size selection and clean-up beads

XpressPure NGS size selection and clean-up beads offer a simple and reliable selection of DNA libraries for different NGS workflows with high recovery comparable to the leading kits available in the market.

How it works:

The system uses MagGenome’s proprietary chemistry involving reversible nucleic acid-binding properties of magnetic beads. This property allows the beads to selectively bind fragments of a specific size range viz 150-800 bp and so eliminates fragment sizes outside this range.

In addition, the binding of the beads to narrow size ranges is based on the use of different sample to bead ratios, thus offering the customer accurate single or double-sided size selection, also assuring a more consistent performance between experiments.

Finally, we have tested the compatibility and performance of XpressPure Size Select beads with different NGS library preparation kits and demonstrated results comparable to the AMPure XP beads.

Product Highlights :

• Efficient clean up and single or double-sided size selection due to highly selective reversible binding.
• Easy substitution into existing bead-based workflows
• Consistent results across different experiments and users
• Fast Magnetic response time and low viscosity
• Automation friendly

Performance and Data

1.Comparison of library profiles from XpressPure and AMPure XP size selection beads

Figure1: Tape station profile of final libraries having expected size distribution of 400-500bp. Libraries were prepared from 300bp Covaris DNA fragments using NEB Next Ultra DNA Library kit (cat#E7370L). AMPure XP beads used in the kit protocol was substituted with XpressPure Size Select beads so that a head-to-head comparison of the performance could be made.

2.Reproducibility across multiple experiments:

Figure 2: XpressPure Size Select Bead showed consistent yield and library size distributions across different experiments as shown in the tape station profile of final library (400bp-500bp) prepared from 300 bp Covaris DNA fragments using NEB Next Ultra DNA Library kit (cat#E7370L).

3.XpressPure performs optimally when used at (1.8x) bead to library ratio.

Figure3: Tape station profile showing XpressPure Size Select bead capture efficiency of Covaris DNA fragment (200 bp) using 1.8x sample to bead ratio ensuring the optimal performance of the bead in this ratio (as recommended by AMPure XP).

Materials supplied

• XpressPure Size Select Beads
• XpressPure Elution Buffer

Materials supplied by the User

• Fresh 80% ethanol (Fresh 80% ethanol should be prepared for optimal results).
• Magnetic stand
• Single or multichannel pipettes and tips
• NGS library preparation kits from any vendor

Handling Recommendations

• Store beads at 4⁰C.
• Store elution buffer at room temperature for optimal results.
• Vortex the beads thoroughly before use so that they are re-suspended well.
• Please allow enough time for beads to come to room temperature before use.