Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerases. It can amplify DNA target up to 2 kb (simple template). The elongation velocity is 1kb/min (70-75°C).
This enzyme possesses 3′ to 5′ exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors.
Contents
Ordering Information
Cat# | Units | Strength | Volume |
PE1604 | 250U | 5U/µl | 50 µl |
PE1605 | 500U | 5U/µl | 100 µl |
PE1606 | 1000U | 5U/µl | 200 µl |
Shipping & Storage Conditions
Product to be shipped and stored at -20°C
Are you looking for a PCR master mix that incorporates this polymerase? Try Pfu DNA Polymerase Master Mix for high-fidelity PCR applications and reliable DNA amplification results.