XpressAutomag Blood DNA Kit

Price range: ₹18,000.00 through ₹230,000.00

XpressAutomag Blood DNA Kit

Price range: ₹18,000.00 through ₹230,000.00

Product Details

XpressAutomag Blood DNA extraction Kit (One-pot method – No Centrifugation involved)

This kit is designed for automated or manual extraction of high-quality DNA from blood with a starting volume of 100-200 μl with an approximate yield of ≥ 5 μg of DNA.

You can use the kit for manual extraction or in automation with the instruments and workstations such as Kingfisher Flex, Zybio and Alta where it is capable of processing 1-96 samples in a single run. The Kit provides patented magnetic nanoparticle-based DNA purification from whole blood samples.

This DNA isolation Kit is based on a direct blood lysis method that consequently involves minimal handling and effective removal of contaminants and inhibitors.

DNA extracted using XpressAutomag Blood DNA Kit is amenable for all downstream applications. You can use the DNA directly in applications such as PCR, Real-time PCR, restriction digestion, and sequencing applications.

Product Specifications 
ParameterSpecifications
Sample TypeFresh/ frozen/ or aged blood samples, blood stored in different vacutainers (EDTA, Heparin, Citrate, and Fluoride)
Instrumentation RequiredAutomation ( Kingfisher Flex, Zybio and Alta etc.) or manual extraction using magnetic separation stand
Sample Volume needed200 μl
Sample run time30-45 minutes
Yield5-30 μg
Samples per run1-96 samples per run.
Elution volume30-100 μl
Downstream applicationsPCR, Real-time PCR, restriction digestion, and sequencing applications.

 

Performance and Data

Extraction of DNA from different blood storage vacutainers

Table 1: Nanodrop data of the gDNA extracted from different blood storage vacutainers

 

StorageConc. (ng/μl)A260/280A260/230Yield (μg)
EDTA118.31.931.855.92
Citrate120.61.931.856.03
Heparin116.21.941.775.81
Fluoride127.71.931.846.39

Blood DNA extraction
Fig 3: Manual protocol using EDTA-stored blood (200ul)

Do also consider trying out our XpressDNA Blood DNA Extraction Kit — ideal for a large variety of blood samples such as stored blood, PBMCs, umbilical cord blood and blood clots. .

> For FTA cards and dried blood spots, you can use our XpressDNA Dried Blood Spot Kit.

Protocol_XpressAutomag Blood DNA Kit

MSDS_XpressAutomag Blood DNA Kit

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