₹4,500.00
2× PfuP Mix is a premixed, ready-to-use solution containing Pfu DNA Polymerase, dNTPs, Mg2+ and Reaction buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. The elongation velocity is 2kb/min (70~75°C). It has 5′ to 3′ polymerase activity and 3′ to 5′ exonuclease activity and results in blunt ended products. The elongation velocity is 1kb/min(70~75°C).
Derived from the hyperthermophilic archae Pyrococcus furiosus. It is a DNA polymerase that has superior thermostability and proofreading properties compared to other thermostable polymerases. It can amplify DNA target up to 2 kb (simple template). The elongation velocity is 1 Kb / min (70-75°C). Pfu DNA polymerase possesses 3′ to 5′ exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors.
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Shipping & Storage Conditions
2X PfuP Mix must be shipped and stored at -20°C
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