XpressDNA Plasmid Kit allows rapid and efficient isolation of plasmid DNA from gram negative bacteria. This Plasmid DNA kit uses our unique and reliable patented magnetic nanoparticles-based technology.
Researchers can isolate low, medium and high copy number plasmids with high purity and negligible genomic DNA contamination.
You can complete the protocol within 30-35 minutes.
A 1.5 ml overnight culture of gram negative bacteria with OD600 ≦ 1 can give a yield of 2-12ug of plasmid DNA with our kit. The extracted plasmid is suitable for use in downstream applications like PCR, restriction enzyme digestion, cloning, transformation, transfection etc.
The XpressDNA Plasmid kit is shipped at 4ºC and on arrival XT buffer and MagNa mix should be stored at 4ºC. All other solutions should be kept tightly sealed and stored at room temperature.
Protocol: