FAQs

Frequently Asked Questions

Blood Mini Kit

What can I do to increase the DNA yield?

Make sure that you use the recommended volume of properly stored blood for extraction. Also ensure that the right amount of Lysis buffer and Proteinase K are added.

Why is the purified genomic DNA sheared?
  • The genomic DNA was handled improperly – Pipetting steps should be handled as gently as possible.
  • Improper storage of sample – Avoid using blood which is clotted or stored at suboptimal conditions.
How would I know if there is ethanol in the extracted DNA?
  • The eluted DNA smells ethanol.
  • DNA floats back from the well of agarose gels even if the DNA is loaded with loading dye.
  • DNA will not freeze well in -20 ℃
How to quantify the extracted DNA?

You can measure DNA by NanoDrop spectrophotometry or by fluorimetry using Qubit. In Qubit assay, the dye binds specifically to double-stranded DNA and not to nucleotides, single-stranded DNA, or RNA.

Why do Qubit and NanoDrop Results Differ?

Qubit assay specifically measures double-stranded DNA and therefore provides a more accurate detection of DNA concentration and amount. NanoDrop spectrophotometry measures DNA concentration by determining the absorbance of light at 260 nm. Though double-stranded DNA has an absorbance at this wavelength, other molecules also absorb including proteins, RNA, single-stranded DNA, free nucleotides, phenol, and other contaminants. Therefore, NanoDrop measurements are prone to overestimation of DNA amounts due to the detection of contaminants in the sample.

What type of blood samples work with XpressDNA Blood Mini Kit?

The XpressDNA Blood Mini kit works well with fresh, old and /or haemolysed blood. The kit is ideal for isolating genomic DNA from blood samples stored in EDTA K2/K3, Heparin and Sodium fluoride storage vials.

Can we use the XpressDNA Blood mini kit for DNA extraction from blood clots?

No. This kit is not recommended for DNA extraction from blood clots.

Can I elute the DNA in 10 mM Tris-Cl, pH 8.0?

Yes. Avoid using Tris-EDTA (TE) buffer as EDTA will inhibit the activity of enzymes used in downstream process such as PCR, restriction digestion, etc.

What are the advantages over existing products /technologies in the market?

The XpressDNA Blood kits are produced using a magnetic nanoparticles based patented technology. The major advantages include lesser dependence on time consuming steps like centrifugation, use of spin columns and multiple tube changes. The recovery of genomic DNA is higher because of the increased surface area of the nanoparticles.

What is the stability of the kit components?

The reagents present in this kit are guaranteed to be stable over a period of one year.

Glutathione-Magnetic Nanoparticles

What is the maximum binding capacity of Glutathione-magnetic nanoparticles?

The maximum binding capacity of Glutathione-magnetic nanoparticles is approximately 2mg of GST-fusion protein per ml of the product.

In what pack sizes the product is available?

Glutathione-magnetic nanoparticles are currently available as 1ml and 5ml products.

What are the advantages of the product over existing products/technologies?

MagGenome’s Glutathione-Magnetic nanoparticles have significantly higher binding capacity compared to most of the Glutathione-magnetic beads available in the market. The protocol is quick and simple because all washing and elution steps are performed with the help of an external magnetic field. It is ensured that the loss of the affinity resin is nil during the process compared to Sepharose beads. Non-specific protein binding is also significantly low.

How is the stability of the product?

The recommended storage condition of the product is 4℃. The product is stable for one year under ideal storage conditions.

What are the applications tested for the product?

Purification of GST tagged fusion proteins.

Protein A-Magnetic Nanoparticles

How do we choose between Protein A and Protein G?

Protein A and Protein G can bind to IgGs, but their binding properties differ among species and subclasses of IgG.
-Protein A is preferred for human, rabbit, pig, dog, and cat IgG.
-Protein G has the better binding capacity for a broader range of mouse, rat and human IgG subclasses.

What is the maximum binding capacity of Protein A-magnetic nanoparticles?

The maximum binding capacity of Protein A-magnetic nanoparticles is approximately 150μg IgG per 1 mg of the nanoparticles.

In what pack sizes the product is available?

Protein A-magnetic nanoparticles are currently available as 1ml and 5ml products.

What are the advantages of the product over existing products/technologies?

MagGenome’s Protein A-Magnetic nanoparticles have significantly higher binding capacity compared to most of the Protein A-magnetic beads available in the market. The protocol is quick and simple because all washing and elution steps are performed with the help of an external magnetic field. It is ensured that the loss of the affinity resin is nil during the process unlike Sepharose beads.

How is the stability of the product?

The recommended storage condition of the product is 4 ℃ .The product is stable for one year under ideal storage conditions.

What are the applications tested for the product?

Small scale antibody purification, Immunoprecipitation, Chromatin immunoprecipitation, RNA-IP.

Protein G-Magnetic Nanoparticles

How do we choose between Protein A and Protein G?

Protein A and Protein G can bind to IgGs, but their binding properties differ among species and subclasses of IgG.
-Protein A is preferred for human, rabbit, pig, dog, and cat IgG.
-Protein G has a better binding capacity for a broader range of mouse, rat, and human IgG subclasses.

What is the maximum binding capacity of Protein G-magnetic nanoparticles?

The maximum binding capacity of Protein G-magnetic nanoparticles is approximately 120-150μg IgG per 1 mg of the nanoparticles.

In what pack sizes the product is available?

Protein G-magnetic nanoparticles are currently available as 1ml and 5ml products.

What are the advantages of the product over existing products/technologies?

MagGenome’s Protein G-Magnetic nanoparticles have significantly higher binding capacity compared to most of the Protein G-magnetic beads available in the market. The protocol is quick and simple because all washing and elution steps are performed with the help of an external magnetic field. It is ensured that the loss of the affinity resin is nil during the process compared to Sepharose beads.

How is the stability of the product?

The recommended storage condition of the product is 40 ℃. The product is stable for one year under ideal storage conditions.

What are the applications tested for the product?

Small scale antibody purification, Immunoprecipitation, Chromatin immunoprecipitation, RNA-IP

Tissue/Cell line Mini Kit

What can I do to increase the DNA yield?

Make sure the appropriate amount of tissue is used for extraction. The tissue has to be completely homogenized. Also ensure that the right amount of Lysis buffer and Proteinase K are added.

Why is the purified genomic DNA sheared?
  • The genomic DNA was handled improperly – Pipetting steps should be handled as gently as possible.
  • Improper storage of sample – Repeated freezing and thawing of stored samples should be avoided as this may lead to shearing. Follow the storage conditions recommended in the user’s manual.
  • The sample is old – Sheared DNA may be obtained from the old tissue or cell samples. Fresh samples are recommended for maximum genomic DNA yield
How would I know if there is ethanol in the extracted DNA?
  • The eluted DNA smells ethanol.
  • DNA floats back from the well of agarose gels even if the DNA is loaded with loading dye.
  • DNA will not freeze well in -20℃
How to quantify the extracted DNA?

You can measure DNA by NanoDrop spectrophotometry or by fluorimetry using Qubit. In Qubit assay, the dye binds specifically to double-stranded DNA and not to nucleotides, single-stranded DNA, or RNA.

Why do Qubit and NanoDrop Results Differ?

Qubit assay specifically measures double stranded DNA and therefore provides a more accurate detection of DNA concentration and amount. NanoDrop spectrophotometry measures DNA concentration by determining the absorbance of light at 260 nm. Though double stranded DNA has the absorbance at this wavelength, other molecules also absorb including proteins, RNA, single stranded DNA, free nucleotides, phenol, and other contaminants. Therefore, NanoDrop measurements are prone to overestimation of DNA amounts due to the detection of contaminants in the sample.

What type of tissue samples work with XpressDNA Tissue/Cell line Mini Kit?

So far, we and our customers have successfully extracted and amplified DNA from animal liver, spleen, kidney, heart, lungs, muscle, rat tail, fish muscle and ethanol preserved fish tissues. Also this kit is ideal for extracting DNA from human tumor tissues and cell lines.

Can I use the extracted DNA for library preparation for next generation sequencing?

Yes, the DNA can be used for library preparation and sequencing. The protocol is optimized to extract DNA without contaminating proteins and PCR inhibitors.

Can I elute the DNA in 10 mM Tris-Cl, pH 8.0?

Yes. Avoid using Tris-EDTA (TE) buffer as EDTA will inhibit the activity of enzymes used in a downstream process such as PCR, restriction digestion, etc.

Can we use the XpressDNA Tissue/Cell line mini kit for DNA extraction from FFPE tissue?

No. This kit is not recommended for DNA extraction from FFPE samples.

What are the advantages over existing products /technologies in the market?

The XpressDNA tissue/cell line kits are produced using magnetic nanoparticles based patented technology. The major advantages include lesser dependence on time-consuming steps like centrifugation, use of spin columns and multiple tube changes. The recovery of genomic DNA is higher because of the increased surface area of the nanoparticles.

What is the stability of the kit components?

The reagents present in this kit are guaranteed to be stable over a period of one year.

Custom Order for protein immobilization on magnetic nanoparticles




Customer Information:


Note: Once the project enquiry is submitted, we will provide an appropriate quotation within 2-3 days. The completion of the project would take 5-7 days from the receipt of protein at our facility and will be shipped from here within that time frame. The specifications of the immobilized product will be
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Product : XpressDNA Tissue/Cell line Mini Kit

25 Reactions


Cat. No : 1404-TI-25

50 Reactions


Cat. No : 1404-TI-50

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Contact Info

Mailing Address

MagGenome Technologies Pvt. Ltd Kolavelil Building, Kunnumpuram, Kakkanad, Cochin-682 030

Email

info@maggenome.com
support@maggenome.com
sales@maggenome.com

Phone

+91 484 2413397
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