XpressDNA Mycobacterium Kit is optimized for genomic mycobacterial DNA extraction, providing a simple and efficient method of genomic DNA purification/extraction from Mycobacterium cells. Magnetic Nanoparticles provide large surface area and highly specific nucleic acid binding capacity resulting in purification of high quality and quantity of double stranded genomic DNA from various bacterial strains. The protocol does not require phenol/chloroform or alcohol precipitation. The purified DNA is approximately 20 – 40 kb in size with highest integrity, double strand ratio and is suitable for downstream applications like NGS, Southern blot, SNP genotyping and PCR or other enzymatic reactions without any further purification.

• Contamination free final product
• Ready to go for sequencing without further purification
• Limited centrifugation steps
• No phenol or chloroform usage
• Wide compatibility with a variety of mycobacterium strains and mycobacterium
• Non-toxic and easy storage of kit reagents

• Mycobacterium Enzyme buffer
• Mycobacterium Lysis buffer
• Mycobacterium MagNa Mix
• Mycobacterium Wash Buffer 1
• Mycobacterium Wash Buffer 2
• Mycobacterium Elution Buffer
• Proteinase K Enzyme lyophilized
• Proteinase K dilution buffer
• Mycobacterium RNase A solution
• MagNa stand (optional)

The XpressDNA Mycobacterium Kit is shipped at room temperature. The enzymes (Proteinase K and RNase) has to be stored at 4°C. All other solutions should be kept tightly sealed and stored at room temperature.