Metagenomics Study and NGS analysis using DNA Extraction Method
Metagenomics is the study of genetic material recovered directly from environmental samples and metagenomic approaches are a growing branch of science and have many applications in different fields. Metagenomics seems to be the ideal culture-independent technique for unravelling the biodiversity of soils and to study how this biodiversity is affected with continuously changing conditions.
The emergence of several next-generation sequencing (NGS) strategies enriched metagenomics. The combination between NGS and metagenomic approaches helped the investigators resolve several issues regarding the microbial diversity and the functions and relationships among different microbial flora.
Conventional methods of studying microbiomes are extremely lossy – most microbes cannot be cultured by plating due to many factors that we are not quite aware of.
The traditional workflow consisted of collecting the sample, plating it, and sequencing what grows – the main limitation being you can only sequence what grows. Current technology allows us to completely bypass the culturing step and instead extract nucleic acids directly from a sample, granting access to theoretically 100% of the genetic content in the sample.
The development of next-generation sequencing (NGS) techniques provides high-throughput sequence analysis with the ability to simultaneously and independently sequence billions of DNA molecules.
Metagenomic analysis is a sophisticated process and involves several steps. Of these steps, a suitable DNA extraction protocol should be adopted to cope with the different chemical and physical characteristics of each sample. Therefore, optimization and comparison between different extraction methods are usually required for each type of sample. The extracted DNA is used to construct the DNA library. This is usually achieved by connecting specific adaptors to one or both ends of the DNA fragments. The reason for utilizing the DNA adaptor is to deal with the pool of samples and then connect them to its original sample. Handling DNA at this stage should be careful to avoid chemical, physical, or enzymatic damage of DNA molecules.
Therefore, for the hassle – free extraction, the DNA can be isolated using our “XpressDNA” kits which has a patented magnetic nanoparticle technology.
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