RNase A is an endoribonuclease that attacks at the 3’ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. RNase A can be inhibited by alkylation of His12 or His119, which are present in the active site of the enzyme. Activators of RNase.
Concentration: 10 mg/ml
- It is an endoribonuclease that specifically cleaves single stranded RNA. It cleaves 3′ end of unpaired C and U residues.
- RNase A specifically cleaves single-stranded RNA at 3′ phosphate linkages of pyrimidine residues leaving pyrimidine 3′ phosphates and oligonucleotides with terminal pyrimidine 3′ phosphates.
- It is used in plasmid and genomic DNA protocols to obtain RNA-free DNA. It is also used for cleaving of unhybridized regions of RNA from DNA-RNA or RNA Hybrids.
Shipping and Storage condition:
The product as supplied is stable at 2-8°C. For long term storage at -20°C.
- Molecular Weight: 13,700 Da
- Optimum pH: 5.0 – 6.0