XpressAutomag Saliva DNA Kit provides patented magnetic nanoparticle-based DNA purification from saliva samples. The kit is designed to isolate high-quality DNA from saliva with a starting volume of 200 μl with an approximate yield of ≥5 μg of DNA. The purification process does not involve phenol/chloroform extraction or alcohol precipitation. XpressAutomag Saliva DNA Kit is based on a direct saliva lysis method that involves minimal handling and effective removal of contaminants and inhibitors. DNA extracted using XpressAutomag Saliva DNA Kit is amenable for all downstream applications such as PCR, Real-time PCR, restriction digestion, and sequencing applications. The kit can be easily adapted to automated magnetic- based separation instruments and workstations.

Product highlights

• Isolate high-quality gDNA from fresh/ frozen/ or stored saliva samples
• Fast, reproducible, and easy processing using magnetic bead based system
• No centrifugation involved
• Isolate gDNA from saliva stored in different stabilization buffer and fresh samples
• Recovered gDNA is compatible with various downstream applications
• Automation friendly with KingFisher Flex, Duo, ZYBIO, Himedia and others

Performance and Data

Extraction of DNA from different saliva samples.

Table 1: PILOT RUN Nanodrop readings of Automation saliva DNA extraction

Fig 1: 1% Agarose gel electrophoresis profile of Genomic DNA from saliva samples