RNase A is an endoribonuclease that attacks at the 3’ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. RNase A can be inhibited by alkylation of His12 or His119, which are present in the active site of the enzyme. Activators of RNase.

Concentration: 10 mg/ml

1. It is an endoribonuclease that specifically cleaves single stranded RNA. It cleaves 3′ end of unpaired C and U residues.
2. RNase A specifically cleaves single-stranded RNA at 3′ phosphate linkages of pyrimidine residues leaving pyrimidine 3′ phosphates and oligonucleotides with terminal pyrimidine 3′ phosphates.
3. It is used in plasmid and genomic DNA protocols to obtain RNA-free DNA. It is also used for cleaving of unhybridized regions of RNA from DNA-RNA or RNA Hybrids.

The product as supplied is stable at 2-8°C. For long term storage at -20°C.

Properties: Molecular Weight: 13,700 Da
Optimum pH: 5.0 – 6.0